Pine strawberry (Fragaria × ananassa Duch.) is one of the most popular fruit crops grown in the temperate zone of the Northern hemisphere. This culture is propagated traditionally vegetative by means of rosettes. For remontant varieties this method is less effective because plants form only 1–2 rosettes per plant during the growing season. The micropropagation in vitro is the alternative method of reproduction to vegetative propagation. The peculiarities of clonal micropropagation pine strawberry have been studied. Three clonal genotypes — gMD1, gMD2 and gMD3 of Moscow Delicatessen cultivar were the object of investigation. For propagation the microrosettes long time strawberry subculture in vitro were taken. Microrosettes were cultivated in agar and sucrose nutrient medium Murashige — Skoog supplemented with growth regulators. The influence of 6-benzylaminopurine (6 BAP) concentration on the coefficient of multiplication as well as the influence of β-indolebutyric acid (IBA) on rooting formation of used strawberry Moscow Delicatessen genotypes in vitro culture have been studied. The optimally low concentrations of 6-benzylaminopurine (0,5– 2,0 μM) on the multipropagation stage and the same for β-indolebutyric acid (0,5–3,0 μM) on the rooting stage have been determined.