The intestine microecology is characterized by the presence of bile produced in the liver cells. Besides its main functions associated with digestion, bile (more exactly, bile acids contains in it) prevents adhesion of the exogenous microbes to the intestinal epithelium. Strains Lactobacillus acidophilus are quite resistant to gall and have adapted to this ecological niche. For example, in contrast to the strains of the Bulgarian sticks, unable to colonize the intestine, they sustain the presence of bile salts in concentration 20% in the nutrient medium. The article presents comparative data on bile tolerance of the collection strains Lactobacillus acidophilus (from the collection of Microbiology laboratory of Siberian cheesemaking research Institute, Barnaul) which are promising for inclusion to the probiotic dairy products. The most resistant to high concentrations of bile and the weakest strains Lactobacillus acidophilus have been identified. Also the basic promising and widely used practical importance of Lactobacillus acidophilus are discussed in the article. Possible advantages and disadvantages of using acidophilic bacteria in various sectors, the quality of acidophilic products and drugs are shown.