The influence of the light type — the white and the blue light — on the concentration of photosynthetic pigments in potato leaves cultivated in vitro has been investigated. As the objects of research potato varieties, recommended for cultivation in the Western Siberia region were used: Kuznechanka, Red Scarlett, Adretta. The concentration of photosynthetic pigments chlorophylls a, b and carotinoids was examined using the spectrophotometry method. Calculation of chlorophylls a and b concentrations was made by the Vernon formula, calculation of carotinoids concentration led by the Wettstein formula. Increased synthesis of photosynthetic pigments indicates a high plasticity of plants that contributes to a better plant adaptation under the different conditions of cultivation. The obtained data indicate sort specific plant responses to the type of lighting variety. It was revealed that growing under the blue light illumination is the best option for the Adretta. The obtained data can be used for recommendations in the lighting type selection, ensuring optimal development of regenerated plants, and providing the adaptation stage to non-sterile conditions.